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Plasma apoAV levels are markedly elevated in severe hypertriglyceridemia and positively correlated with the APOA5 S19W polymorphism

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Author: Henneman, P. · Schaap, F.G. · Havekes, L.M. · Rensen, P.C.N. · Frants, R.R. · Tol, A. van · Hattori, H. · Smelt, A.H.M. · Dijk, K.W. van
Type:article
Date:2007
Institution: TNO Kwaliteit van Leven
Source:Atherosclerosis, 1, 193, 129-134
Identifier: 240057
doi: doi:10.1016/j.atherosclerosis.2006.05.030
Keywords: Biology · Biomedical Research · apoAV · Hypertriglyceridemia · Lipoprotein lipase · Plasma apoAV concentration · SNP-analysis · apolipoprotein A5 · adult · allele · article · circulation · controlled study · endoplasmic reticulum · female · gene frequency · gene mutation · genetic polymorphism · genetic variability · haplotype · human · hypertriglyceridemia · lipolysis · major clinical study · male · priority journal · protein secretion · single nucleotide polymorphism · Adult · Alleles · Apolipoproteins A · Base Sequence · Case-Control Studies · DNA Primers · Female · Gene Frequency · Haplotypes · Humans · Hypertriglyceridemia · Male · Middle Aged · Polymorphism, Single Nucleotide

Abstract

Objective: The recently discovered apoAV is hypothesized to affect triglyceride metabolism by stimulating the lipolysis of triglycerides in VLDL and chylomicrons. We set out to determine the association between increased serum TG levels, plasma apoAV levels, and polymorphism of the APOA5 gene, with specific emphasis on the APOA5 S19W variation. This mutation alters the endoplasmic reticulum signal peptide and is hypothesized to impair apoAV secretion into the circulation. Methods and results: Two haplotype-tagging APOA5 polymorphisms, APOA5 S19W and APOA5 -1131T > C and plasma apoAV levels were determined in a population of patients with severe hypertriglyceridemia (HTG). As compared to a random control population, the allele frequencies of the APOA5 S19W and -1131T > C rare variants were significantly increased in HTG patients. Furthermore, the HTG population exhibited markedly elevated plasma apoAV levels that were positively correlated with serum TG levels. Plasma apoAV levels were positively correlated with occurrence of the APOA5 S19W rare variant. Conclusions: The increased allele frequencies of the APOA5 S19W and -1131T > C rare variants in the HTG population are in agreement with previous reports. Our data show a positive correlation between apoAV and TG levels. Moreover the finding of a positive association between apoAV levels and the APOA5 S19W rare variant is in disagreement with the hypothesis that this variant is poorly secreted. © 2006 Elsevier Ireland Ltd. All rights reserved.