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Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry

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Author: Trip, H. · Mende, K. · Majchrzykiewicz-Koehorst, J.A. · Sedee, N.J.A. · Hulst, A.G. · Jansen, H.J. · Murray, C.K. · Paauw, A.
Type:article
Date:2015
Publisher: American Society for Microbiology
Source:Journal of Clinical Microbiology, 6, 53, 1927-1930
Identifier: 525581
doi: doi:10.1128/JCM.00620-15
Keywords: Biology · Acinetobacter baumannii · Observation, Weapon & Protection Systems · CBRN - CBRN Protection · TS - Technical Sciences

Abstract

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing. Copyright © 2015, American Society for Microbiology. All Rights Reserved.