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A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

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Author: Argnani, A. · Leer, R.J. · Luijk, N. van · Pouwels, P.H.
Institution: TNO Voeding
Source:Microbiology, 1, 142, 109-114
Identifier: 233155
Keywords: Nutrition · Electrotransformation · Plasmid vector · Plasmid dna · Bacterium competence · Vontrolled study · Expression vector · Genetic transformation · Lactobacillus bifidus · Methodology · Nonhuman · Priority journal · Amino Acid Sequence · Bifidobacterium · Corynebacterium · DNA Replication · Electroporation · Genes, Bacterial · Genetic Vectors · Lactobacillus · Lactococcus · Molecular Sequence Data · Plasmids · Reproducibility of Results · Sequence Homology, Amino Acid · Species Specificity · Transformation, Genetic


A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4°C. Transformation of Bifidobacterium could be achieved with a plasmid vector originating from Bifidobacterium and with plasmid vectors from Corynebacterium, but not with vectors carrying replicons from Lactococcus or Lactobacillus.