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The effect of X-rays on the precursors of antibody forming cells (B cells) as measured with the in vitro limiting dilution assay

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Author: Lubbe, F.H. · Hooijkaas, H. · Preesman, A.A.
Institution: Medisch Biologisch Laboratorium TNO
Source:International Journal of Radiation Biology, 2, 42, 131-139
Identifier: 229229
Keywords: Biology · B lymphocyte · Cell culture · In vitro study · Lymphoblast · Mouse · Spleen · Spleen cell · X ray · Animal · Antibody-Producing Cells · B-Lymphocytes · Cell Count · Female · Immunoglobulin G · Immunoglobulin M · In Vitro · Lipopolysaccharides · Lymphocyte Activation · Mice · Mice, Inbred C57BL · Rats · Rats, Inbred Lew · Specific Pathogen-Free Organisms · Support, Non-U.S. Gov't


The effect of X-irradiation upon murine antibody-forming cell precursors (B cells) was established in cultures of spleen cells stimulated with the B cell mitogen lipopolysaccharide (LPS). At day 5 and 7 the numbers of IgM- and IgG2-secreting cells were determined in cultures of irradiated and nonirradiated spleen cells. From these numbers a D(o) of 0.6-1.2 Gy for the IgM, and of 0.9-2.1 Gy for the IgG2 response was calculated. Similar D(o) values were obtained under limiting dilution culture conditions. In the limiting dilution assay the effect of irradiation upon the size of the IgM-producing clones could also be determined. It was found that irradiation reduced the number of LPS-reactive B cells without affecting the size of the clones produced by the surviving cells. Chemicals/CAS: Immunoglobulin G; Immunoglobulin M; Lipopolysaccharides