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Complications in the determination of HDL2/HDL3 ratios

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Author: Kremer, J.M.H. · Havekes, L.
Institution: Gaubius instituut TNO
Source:Clinica Chimica Acta, 1, 109, 21-29
Identifier: 228976
doi: doi:10.1016/0009-8981(81)90132-7
Keywords: High density lipoprotein · Centrifugation, Density Gradient · Electrophoresis, Polyacrylamide Gel · Human · Lipoproteins, HDL


We investigated different procedures to subfractionate high density lipoprotein (HDL) into its density subfractions (HDL2 and HDL3). When pore-limit as well as zone polyacrylamide gel electrophoresis was used, it proved to be necessary to use freshly prepared and plasma protein-free HDL preparations. For a quantitative subfractionation of HDL in HDL2 and HDL3 we also tested density gradient ultracentrifugation. To avoid interference with LDL contamination a low starting density was required. Both methods, electrophoresis as well as density gradient ultracentrifugation were too laborious and time-consuming for large scale use. We devised a combination of both ultracentrifugation and electrophoresis as a method which is relatively appropriate for determinations of HDL2/HDL3 ratios on large scale. Chemicals/CAS: high density lipoprotein-2; high density lipoprotein-3; Lipoproteins, HDL