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Determination of daminozide in apples and apple leaves by liquid chromatography-mass spectrometry

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Author: Mol, H.G.J. · Dam, R.C.J. van · Vreeken, R.J. · Steijger, O.M.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Chromatography A, 1, 833, 53-60
Identifier: 234944
doi: doi:10.1016/S0021-9673(98)00915-7
Keywords: Nutrition · Apples · Daminozide · Food analysis · Fruits · Daminozide · Apple · Conference paper · Controlled study · Drug determination · Food analysis · Growth regulation · Liquid chromatography · Methodology · Nonhuman · Priority journal · Tandem mass spectrometry · Chromatography, Liquid · Fruit · Mass Spectrometry · Plant Growth Regulators · Sensitivity and Specificity · Succinates · Lonchocarpus capassa · Malus x domestica


A straightforward and efficient method was developed for the determination of intact daminozide in apples and apple leaves. After extraction with methanol and a clean-up step using a graphitized carbon cartridge, the extract was analysed by ion-trap liquid chromatography-tandem mass spectrometry (LC-MS-MS) using atmospheric pressure chemical ionisation in the positive ion mode. Recoveries for apple were 98-102% with a R.S.D.≤11% (n=6) and for leaves were 112-116% with a R.S.D.≤18% (n=6). The limits of detection were 0.008 and 0.02 mg/kg for apples and leaves, respectively. Copyright (C) 1999 Elsevier Science B.V.