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Inactivation of Escherichia coli by superoxide radicals and their dismutation products

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Author: Hemmen, J.J. van · Meuling, W.J.A.
Institution: Medisch Biologisch Laboratorium TNO
Source:Archives of Biochemistry and Biophysics, 2, 182, 743-748
Identifier: 228247
Keywords: Biology · Catalase · Dialuric acid · Edetic acid · Hydrogen peroxide · Oxygen · Superoxide · Superoxide dismutase · Escherichia coli · In vitro study · Microorganism · Theoretical study · Catalase · Edetic Acid · Escherichia coli · Free Radicals · Hydrogen Peroxide · Kinetics · Mathematics · Oxygen · Superoxide Dismutase · Superoxides


E. coli cells are inactivated by the products of the reaction between dialuric acid and oxygen, of which the primary product is superoxide. The rate of inactivation is decreased by superoxide dismutase, by catalase, and by EDTA, whereas it is increased by addition of cupric ions or hydrogen peroxide. It is concluded that a toxic product is formed in a reaction involving superoxide, hydrogen peroxide, and metal ions, which might be the Haber-Weiss reaction, O2- + H2O2 → OH + OH- + O2. In radiation chemical experiments it is shown that this reaction does not occur in the absence of metal ions. Chemicals/CAS: catalase, 9001-05-2; dialuric acid, 444-15-5; edetic acid, 150-43-6, 60-00-4; hydrogen peroxide, 7722-84-1; oxygen, 7782-44-7; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; superoxide, 11062-77-4; Catalase, EC; Edetic Acid, 60-00-4; Free Radicals; Hydrogen Peroxide, 7722-84-1; Oxygen, 7782-44-7; Superoxide Dismutase, EC; Superoxides, 11062-77-4