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Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract

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Author: Braakhuis, B.J.M. · Welters, M.J.P. · Cloos, J. · Pankras, J.E. · Smeets, S.J. · Fichtinger-Schepman, A.-M.J.
Type:article
Date:1999
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Cancer Letters, 1-2, 138, 217-220
Identifier: 235012
doi: doi:10.1016/S0304-3835(99)00016-6
Keywords: Nutrition · Cisplatin · Head and neck · Keratinocytes · Non-malignant cells · Pt accumulation · Squamous cells · Cisplatin · Platinum · Adult · Antineoplastic activity · Cancer cell culture · Colorimetry · Controlled study · Drug accumulation · Epithelium cell · Female · Growth inhibition · Head and neck cancer · Human · Human cell · Keratinocyte · Male · Palate · Priority journal · Squamous cell · Squamous epithelium · Antineoplastic Agents · Carcinoma, Squamous Cell · Cell Division · Cisplatin · Epithelial Cells · Female · Head and Neck Neoplasms · Humans · Male · Middle Aged · Platinum · Tumor Cells, Cultured · Uvula

Abstract

The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.