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Transcription factor NF-kB as a potential biomarker for oxidative stress

Author: Berg, R. van den · Haenen, G.R.M.M. · Berg, H. van den · Bast, A.
Type:article
Date:2001
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:British Journal of Nutrition, suppl. 1, 86, S121-S127
Identifier: 57159
Keywords: Nutrition · Biological Markers · Cell Line · Cell Nucleus · Electrophoresis, Polyacrylamide Gel · Hela Cells · Humans · Leukocytes, Mononuclear · NF-kappa B · Oxidative Stress · Sarcoidosis · Antioxidants · Reactive oxygen species

Abstract

There is increasing interest in the involvement of transcription factors, such as of the transcription factor NF-κB (nuclear factor-κB), in the pathogenesis of various diseases. NF-κB is involved in the control of the transcription of a variety of cellular genes that regulate the inflammatory response by the production of cytokines, chemokines, cell adhesion molecules and acute phase proteins. The involvement of NF-κB is especially of interest as it is activated by oxidative stress and its activation can be modulated by antioxidant compounds. The activation of NF-κB can be determined by the electromobility shift assay (EMSA) with a NF-κB binding-site-specific probe. EMSA can also be used on human mononuclear cells isolated from peripheral blood, which could make the assay applicable for clinical trials. The critical steps of the EMSA are discussed, addressing some pitfalls of the assay. The procedure that can be used to express NF-κB activity in human subjects is evaluated. This offers the possibility to use NF-κB as a functional biomarker of oxidative stress as illustrated by several examples of in vitro and in vivo studies. Chemicals/CAS: Biological Markers; NF-kappa B