Repository hosted by TU Delft Library

Home · Contact · About · Disclaimer ·

A low-fat diet has a higher potential than energy restriction to improve high-fat diet-induced insulin resistance in mice

Publication files not online:

Author: Muurling, M. · Jong, M.C. · Mensink, R.P. · Hornstra, G. · Dahlmans, V.E.H. · Pijl, H. · Voshol, P.J. · Havekes, L.M.
Institution: Gaubius Instituut TNO
Source:Metabolism: Clinical and Experimental, 6, 51, 695-701
Identifier: 236575
doi: doi10.1053/meta.2002.32725
Keywords: Biology Health · fat · fatty acid · glucose · high density lipoprotein · insulin · intermediate density lipoprotein · low density lipoprotein · triacylglycerol · very low density lipoprotein · animal experiment · animal model · article · body weight · caloric intake · controlled study · energy metabolism · feeding · glucose tolerance · insulin blood level · insulin dependent diabetes mellitus · insulin resistance · insulin sensitivity · lipid diet · lipid metabolism · low fat diet · male · mouse · non insulin dependent diabetes mellitus · nonhuman · obesity · priority journal · Animals · Blood Glucose · Body Weight · Cholesterol · Dietary Fats · Energy Intake · Fatty Acids, Nonesterified · Glucose Tolerance Test · Insulin · Insulin Resistance · Lipids · Lipoproteins · Lipoproteins, VLDL · Liver · Male · Mice · Mice, Inbred C57BL · Polyethylene Glycols · Triglycerides


Previous studies have shown that energy restriction (ER) or low-fat (LF) diets have beneficial effects on high-fat (HF) diet-induced obesity and non-insulin-dependent diabetes. However, comparison between ER and low-fat diet regarding the effect on insulin resistance and lipid metabolism has not been reported. After inducing insulin resistance by HF feeding for 20 weeks, male C57BL/6J mice were divided into 3 groups. For a period of 12 weeks, group 1 received energy restriction (70% of ad libitum, HF diet), group 2 LF diet, and group 3 maintained on HF diet. Body weight and energy intake were reduced equally in ER and LF feeding. Plasma insulin levels were decreased on LF feeding, but were unchanged on ER, when compared with HF feeding. Glucose tolerance and insulin sensitivity tests revealed that insulin sensitivity was improved more efficiently by LF feeding than on ER. Plasma triglyceride (TG) levels were lower on LF feeding compared with ER and HF feeding. Measurement of hepatic very-low-density lipoprotein (VLDL)-TG production revealed a lower production after LF diet feeding or ER compared with HF diet feeding. In summary, our data show that LF diet has a higher potential than ER to improve HF diet-induced insulin resistance, and that there is an association between improvement of insulin resistance and decrease of TG levels. Copyright 2002, Elsevier Science (USA). All rights reserved.