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Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi

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Author: Gemeren, I.A. van · Musters, W. · Hondel, C.A.M.J.J. van den · Verrips, C.T.
Type:article
Date:1995
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Biotechnology, 40, 155-162
Identifier: 83418
doi: DOI:10.1016/0168-1656(95)00041-N
Keywords: Nutrition · A. awamori · F. solani pisi cutinase · Filamentous fungi · Heterologous expression · S. cerevisiae · Synthetic gene construction · Complementary dna · Cutinase · Aspergillus · Biosynthesis · Controlled study · Fusarium solani · Gene expression · Nonhuman · Priority journal · Saccharomyces cerevisiae · Amino Acid Sequence · Aspergillus · Base Sequence · Biotechnology · Carboxylic Ester Hydrolases · DNA, Complementary · DNA, Fungal · Fusarium · Genes, Fungal · Genes, Synthetic · Genetic Vectors · Molecular Sequence Data · Plasmids · Recombinant Proteins · Saccharomyces cerevisiae · Support, Non-U.S. Gov't · Aspergillus · Aspergillus awamori · Fungi · Fusarium · Fusarium solani · Saccharomyces cerevisiae

Abstract

A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori.