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Reduction of polyneuronal innervation of muscle cells in tissue culture after long-term indirect stimulation

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Author: Magchielse, T. · Meeter, E.
Type:article
Date:1982
Institution: Medisch Biologisch Laboratorium TNO
Source:Developmental Brain Research, 1, 3, 130-133
Identifier: 229177
Keywords: Biology · Tubocurarine chloride · Animal experiment · Chicken · Ciliary ganglion · Electrostimulation · In vitro study · Innervation · Muscle · Muscle cell · Muscle innervation · Nervous system · Neuromuscular transmission · Peripheral nervous system · Tissue culture

Abstract

In combined cultures of chick muscle cells and ciliary ganglia, the effect of indirect, long-term stimulation on the number of neuromuscular contacts on individual muscle cells was studied. Seven days of phasic stimulation resulted in a marked reduction in the number of neuromuscular contacts, the majority of the muscle cells becoming innervated by only one neuron. Neuromuscular blockade by D-tubocurarine prevented this loss of innervation. Chemicals/CAS: tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7