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Effects of vitamin C supplementation in human volunteers with a range of cholesterol levels on biomarkers of oxygen radical-generated damage

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Author: Anderson, D. · Phillips, B.J. · Yu, T-W. · Edwards, A.J. · Ayesh, R. · Butterworth, K.R.
Type:article
Date:2000
Source:Pure and Applied Chemistry, 6, 72, 973-983
Identifier: 41673
Keywords: Medicine · Geneeskunde · Health · Gezondheid · Dietetics · Voedingsleer · ascorbic acid · biological marker · bleomycin · cholesterol · horlicks · oxygen radical · placebo · protein p21 · Ras protein · sanatogen · adult · aged · antioxidant activity · cholesterol blood level · chromosome aberration · clinical trial · comet assay · conference paper · controlled clinical trial · controlled study · crossover procedure · DNA damage · dose response · female · human · human experiment · lipid peroxidation · lymphocyte · male · normal human · oxidative stress · priority journal · sex difference · vitamin blood level · vitamin supplementation

Abstract

Twenty-four men and 24 women, all nonsmoking, and maintaining normal dietary habits were assigned to 3 groups of 16. Each group comprising 4 males with "low" cholesterol levels (<6 mmol/L) matched for age and build with 4 males with "high" cholesterol levels (>6 mmol/L) and 8 similarly matched females. A three-treatment, three-treatment period, cross-over design was adopted. The three treatments were placebo, 60 mg vitamin C/day (the recommended daily allowance) and 6 g vitamin C/day for 14 days with 6 weeks between treatments. Blood samples were taken at the end of each treatment period. Vitamin C supplementation significantly increased plasma vitamin C concentrations and total antioxidant capacity, but did not affect cholesterol status or plasma ras p21 protein levels. There was a nonsignificant dose-related decrease in plasma lipid peroxidation breakdown products. DNA damage, measured in lymphocytes by the Comet assay and chromosome aberration test, was not increased after vitamin C supplementation. Sensitivity to hydrogen peroxide (in the Comet assay) was also unaffected, but sensitivity to chromosome aberration induced by bleomycin was increased by supplementation. A significant gender difference was found in plasma vitamin C levels, antioxidant capacity, and number of chromosome aberrations. Results were independent of low and high cholesterol status.