Repository hosted by TU Delft Library

Home · Contact · About · Disclaimer ·
 

Identification of outer membrane proteins of Yersinia pestis through biotinylation

Publication files not online:

Author: Smither, S.J. · Hill, J. · Baar, B.L.M. van · Hulst, A.G. · Jong, A.L. de · Titball, R.W.
Type:article
Date:2007
Institution: TNO Defensie en Veiligheid
Source:Journal of Microbiological Methods, 1 (January), 68, 26-31
Identifier: 239815
doi: doi:10.1016/j.mimet.2006.05.014
Keywords: Biotinylation · Outer membrane · Proteomics · Yersinia pestis · Bacterial enzyme · Bacterial protein · Biotin · Catalase · Catalase peroxidase · Outer membrane protein · Peroxidase · Streptavidin · Article · Bacterial virulence · Biotinylation · Nonhuman · Plague · Priority journal · Protein analysis · Proteomics · Western blotting · Yersinia pestis · Amino Acid Sequence · Bacterial Outer Membrane Proteins · Biotinylation · Blotting, Western · Electrophoresis, Gel, Two-Dimensional · Plague · Spectrometry, Mass, Electrospray Ionization · Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization · Yersinia pestis · Negibacteria · Yersinia pestis · Biotin, 58-85-5 · Catalase, 9001-05-2 · Peroxidase, 9003-99-0 · Streptavidin, 9013-20-1 · Bacterial Outer Membrane Proteins

Abstract

The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase. Crown Copyright © 2006.