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Lactic acid bacteria as antigen delivery vehicles for oral immunization purposes

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Author: Pouwels, P.H. · Leer, R.J. · Shaw, M. · Heijne Den Bak-Glashouwer, M.J. · Tielen, F.D. · Smit, E. · Martinez, B. · Jore, J. · Conway, P.L.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:International Journal of Food Microbiology, 2, 41, 155-167
Identifier: 234470
doi: DOI:10.1016/S0168-1605(98)00048-8
Keywords: Nutrition · Adherence · Expression vector · Lactobacillus · Surface protein · Vaccine · Antigen · Bacterial protein · Membrane protein · Antigen presentation · Bacterial colonization · Bacterium adherence · Developing country · Drug delivery system · Epithelium cell · Expression vector · Immunization · Immunogenicity · Lactic acid bacterium · Lactobacillus · Protein structure · Review · Structure activity relation · Administration, Oral · Animals · Antigens · Bacterial Adhesion · Colony Count, Microbial · Drug Delivery Systems · Gene Expression Regulation, Bacterial · Genetic Engineering · Humans · Intestinal Mucosa · Lactobacillus · Lactococcus · Mice · Vaccines · Bacteria (microorganisms) · Lactobacillus · Posibacteria


In vaccination programmes in which large numbers of subjects are involved, the oral route of administration is more convenient as compared to the more frequently used parenteral route. This is particularly relevant when vaccines are to be applied in less industrialized countries. Lactic acid bacteria in general and strains of Lactobacillus in particular have a variety of properties which make them attractive candidates for oral vaccination purposes, e.g. GRAS status, adjuvant properties, mucosal adhesive properties and low intrinsic immunogenicity. An overview is given of current research aimed at unravelling the relationship between structure and properties of surface proteins of lactobacilli and in vivo colonization, in particular of species capable of adhering to epithelial cells in vitro. Secondly, the state of the art will be discussed with respect to antigen presentation by lactic acid bacteria. Finally, some preliminary immunological data of recombinant lactic acid bacterial strains expressing antigens from pathogens will be presented.