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Effects of a milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids in male volunteers

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Author: Schaafsma, G. · Meuling, W.J.A. · Dokkum, W. van · Bouley, C.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:European Journal of Clinical Nutrition, 52, 436-440
Identifier: 86039
Keywords: Nutrition · Fructo-oligosaccharides · Lactobacillus acidophilus · Serum cholesterol · Yogurt · Cholesterol · Fructose oligosaccharide · Glucose · High density lipoprotein cholesterol · Low density lipoprotein cholesterol · Milk fat · Triacylglycerol · Vegetable oil · Yoghurt · Adult · Cholesterol blood level · Clinical trial · Controlled study · Crossover procedure · Double blind procedure · Glucose blood level · Human · Human experiment · Lactobacillus acidophilus · Lipid blood level · Male · Milk · Netherlands · Randomized controlled trial · Triacylglycerol blood level · Animals · Apolipoprotein E3 · Apolipoproteins E · Blood Glucose · Cholesterol · Cholesterol, HDL · Cholesterol, LDL · Double-Blind Method · Fermentation · Fructose · Humans · Lactobacillus acidophilus · Lipids · Male · Middle Aged · Milk · Oligosaccharides · Triglycerides · Lactobacillus acidophilus


Objective: To investigate in adult male volunteers the effect of a new fermented milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids. Design: Randomized placebo-controlled double-blind two-way cross over trial with two treatment periods of three weeks, separated by a wash-out period of one week. Setting: the study was performed at the TNO Nutrition and Food Research Institute in Zeist, The Netherlands. Subects: Thirty normal healthy men, aged 33-64 y (mean serum total cholesterol level: 5.23 ± 1.03 (s.d.)), were selected for this study. Normal health was assessed by pre study screening. All subjects were used to an average Dutch food pattern. Interventions: During the treatment periods subjects consumed three times daily a 125 ml of either test or reference product as a part of their habitual diet. The test product was a milk, fermented by yogurt starters and Lactobacillus acidophilus, and contained 2.5% fructo-oligosaccharides, 0.5% vegetable oil and 0.5% milk fat. The reference product was a traditional yogurt (milk fermented only by yogurt strains), containing 1% milk fat. Blood samples for serum lipid analysis and blood glucose measurements were taken before the start of the experiment and at the end of both treatment periods. Results: As compared to the reference product, consumption of the test product resulted in significantly lower values for serum total cholesterol (P < 0.001), LDL-cholesterol (P < 0.005), and the LDL/HDL-ratio (P < 0.05) by 4.4, 5.4 and 5.3% respectively. Levels of serum HDL-cholesterol, triglycerides and blood glucose remained essentially unchanged. The beneficial effects of the test product on serum cholesterol were largely related to an increase of this parameter during the consumption of the reference product. Conclusions: As compared to traditional yogurt, daily consumption of three times 125 ml of test product specifically lowered serum LDL-cholesterol levels in normal healthy male adult subjects with borderline elevated levels of serum total cholesterol within three weeks. Sponsorship: The study was sponsored by Danone, France.