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Animal products and K-ras codon 12 and 13 mutations in colon carcinomas

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Author: Kampman, E. · Voskuil, D.W. · Kraats, A.A. van · Balder, H.F. · Muijen, G.N.P. van · Goldbohm, R.A. · Veer, P. van 't
Type:article
Date:2000
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Carcinogenesis, 2, 21, 307-309
Identifier: 41374
Keywords: Nutrition · Calcium · Protein · Adult · Aged · Allele · Animal · Cancer risk · Case control study · Codon · Colon cancer · Controlled study · Diet · Female · Gene mutation · Human · Major clinical study · Male · Meat · Oncogene K ras · Polymerase chain reaction · Poultry · Priority journal · Statistical analysis · Animals · Calcium, Dietary · Carcinoma · Case-Control Studies · Codon · Colonic Neoplasms · Dairy Products · Dietary Fats · Dietary Proteins · DNA Adducts · DNA Mutational Analysis · DNA, Neoplasm · Female · Fishes · Food Habits · Genes, ras · Humans · Male · Meat · Middle Aged · Mutation · Netherlands · Odds Ratio · Polymerase Chain Reaction · Poultry · Questionnaires

Abstract

K-ras gene mutations (codons 12 and 13) were determined by PCR-based mutant allele-specific amplification (MASA) in tumour tissue of 185 colon cancer patients: 36% harboured mutations, of which 82% were located in codon 12. High intakes of animal protein, calcium and poultry were differently associated with codon 12 and 13 mutations: odds ratios (OR) and 95% confidence intervals (95% CI) for codon 12 versus codon 13 were 9.0 (2.0-42), 4.1 (1.4-12) and 15 (1.4-160), respectively. In case-control comparisons, high intakes of animal protein and calcium were positively associated with colon tumours harbouring codon 12 mutations [for animal protein per 17 g, OR (95% CI) = 1.5 (1.0-2.1); for calcium per 459 mg, 1.2 (0.9-1.6)], while inverse associations were observed for tumours with K-ras mutations in codon 13 [for animal protein 0.4 (0.2-1.0); for calcium 0.6 (0.3-1.2)]. Transition and transversion mutations were not differently associated with these dietary factors. These data suggest a different dietary aetiology of colon tumours harbouring K-ras codon 12 and 13 mutations Chemicals/CAS: Calcium, Dietary; Codon; Dietary Fats; Dietary Proteins; DNA Adducts; DNA, Neoplasm