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Diet-induced obesity increases NF-κB signaling in reporter mice

Author: Carlsen, H. · Haugen, F. · Zadelaar, A.S.M. · Kleemann, R. · Kooistra, T. · Drevon, C.A. · Blomhoff, R.
Institution: TNO Kwaliteit van Leven
Source:Genes and Nutrition, 4, 215-222
Identifier: 241712
doi: doi:10.1007/s12263-009-0133-6
Keywords: Biomedical Research · Glucose intolerance · High-fat diet · Inflammation · Luciferase · Molecular imaging


The nuclear factor (NF)-κB is a primary regulator of inflammatory responses and may be linked to pathology associated with obesity. We investigated the progression of NF-κB activity during a 12-week feeding period on a high-fat diet (HFD) or a low-fat diet (LFD) using NF-κB luciferase reporter mice. In vivo imaging of luciferase activity showed that NF-κB activity was higher in the HFD mice compared with LFD-fed mice. Thorax region of HFD females displayed fourfold higher activity compared with LFD females, while no such increase was evident in males. In male HFD mice, abdominal NF-κB activity was increased twofold compared with the LFD males, while females had unchanged NF-κB activity in the abdomen by HFD. HFD males, but not females, exhibited evident glucose intolerance during the study. In conclusion, HFD increased NF-κB activity in both female and male mice. However, HFD differentially increased activity in males and females. The moderate increase in abdomen of male mice may be linked to glucose intolerance. © 2009 Springer-Verlag.