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Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire

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Author: Noort, J.M. van · Verbeek, R. · Meilof, J.F. · Polman, C.H. · Amor, S.
Type:article
Date:2006
Institution: TNO Kwaliteit van Leven
Source:Multiple Sclerosis, 3, 12, 287-293
Identifier: 239300
doi: doi:10.1191/135248506ms1271oa
Keywords: Health · Biomedical Research · Alpha B-crystallin · Determinant spreading · Alpha crystallin · Autoantibody · Immunoglobulin A · Immunoglobulin G · Immunoglobulin M · Antigen antibody reaction · Cellular immunity · Clinical article · Controlled study · Disease association · Eye inflammation · Immune response · Immunoglobulin blood level · Immunoreactivity · T lymphocyte · Adult · alpha-Crystallin B Chain · Antibody Specificity · Autoantibodies · Autoantigens · Chromatography, High Pressure Liquid · Electrophoresis, Polyacrylamide Gel · Eye Proteins · Female · Humans · Immune System · Lens, Crystalline · Male · Middle Aged · Multiple Sclerosis · Uveitis

Abstract

Human T-cell responses to the stress protein alpha B-crystallin in multiple sclerosis (MS)-affected brain samples are dominant when compared to other myelin antigens. The establishment of the apparent autoimmune repertoire against this antigen has been suggested to involve cross-priming during viral infection. Yet, another possibility would be that determinant spreading during ocular inflammation could generate a response to alpha B-crystallin, since it is also a major component of the eye. In this study, we compared serum IgG, IgA and IgM repertoires against a range of eye lens-derived ocular antigens using sera from healthy control subjects and MS patients with or without uveitis. This comparison revealed that among ocular antigens, alpha B-crystallin is the dominant target antigen for serum autoantibodies in both MS patients and healthy controls. Uveitis generally did not affect the antibody reactivity profile. These data provide further support for the notion that a normal adult human immune system is selectively reactive to alpha B-crystallin and they indicate that this responsiveness is unlikely to result from determinant spreading following ocular inflammation. © 2006 Edward Arnold (Publishers) Ltd. Chemicals / CAS: immunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6; alpha-Crystallin B Chain; Autoantibodies; Autoantigens; Eye Proteins