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A neoantigenic determinant in the D-dimer fragment of fibrin

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Author: Lugovskoy, E.V. · Kolesnikova, I.N. · Gritsenko, P.G. · Zolotareva, E.N. · Gaffney, P. · Nieuwenhuizen, W. · Komisarenko, S.V
Type:article
Date:2002
Institution: TNO Preventie en Gezondheid
Source:Thrombosis Research, 3-4, 107, 151-156
Identifier: 236652
doi: doi10.1016/S0049-3848(02)00204-9
Keywords: Biology · D-dimer · Fibrin · Monoclonal antibody · Neoantigenic determinant · D dimer · epitope · fibrin · fibrinogen · monoclonal antibody · monoclonal antibody III db · unclassified drug · fibrin degradation product · fibrin fragment d · antigen binding · article · blood analysis · controlled study · cross reaction · dissociation constant · enzyme linked immunosorbent assay · human · immunoblotting · methylation · priority journal · protein isolation · serodiagnosis · antibody affinity · epitope mapping · immunology · Antibodies, Monoclonal · Antibody Affinity · Enzyme-Linked Immunosorbent Assay · Epitope Mapping · Epitopes · Fibrin · Fibrin Fibrinogen Degradation Products · Humans · Immunoblotting

Abstract

Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma. © 2002 Elsevier Science Ltd. All rights reserved. Chemicals/CAS: Antibodies, Monoclonal; Epitopes; Fibrin Fibrinogen Degradation Products; fibrin fragment D; Fibrin, 9001-31-4