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Effects of all-trans retinol and cigarette smoke condensate on hamster tracheal epithelium in organ culture. I. A cell proliferation study

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Author: Rutten, A.A.J.J.L. · Wilmer, J.W.G.M. · Beems, R.B.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Virchows Archiv Abteilung B Cell Pathology, 3, 55, 167-175
Identifier: 230515
Keywords: cigarette smoke condensate · retinol · animal cell · animal experiment · cell growth · histology · nonhuman · organ culture · squamous cell metaplasia · trachea mucosa · ultrastructure · Animal · Cell Division · Dimethyl Sulfoxide · Epithelium · Hamsters · Mesocricetus · Metaplasia · Organ Culture · Plants, Toxic · Smoke · Tobacco · Trachea · Vitamin A · Vitamin A Deficiency


The effects of cigarette smoke condensate (CSC) and all-trans retinol on the cell proliferative activity of vitamin A-deprived hamster tracheal epithelium have been studied in vitamin A-deficient, serum-free, hormone-supplemented medium in organ culture. In the absence of retinol, CSC induced a dose-dependent increase in labelig index (LI) during 12 days of culture. The basal cells were more sensitive to CSC exposure than non-basal cells during the first 6 to 8 culture days. However, in squamous metaplastic foci developing after culture day 6, both basal and non-basal cells in the mid-part of the epithelium were labeled. Physiological concentrations of all-trans retinol stimulated the non-basal LI and inhibited the basal cell LI. Compared with dimethylsulfoxide (DMSO), all retinol concentrations used in the present study inhibited the basal cell LI at each time point examined (4-12 days culture). Exposure of tracheal rings to retinol, either before or after exposure to CSC, or simultaneous exposure to retinol and CSC, clearly decreased the CSC-induced basal cell proliferative activity depending on the retinol concentration used. It is concluded from the present study that squamous metaplasia induced by vitamin A-deficiency or by CSC originates mainly from basal cells and that for the maintenance of these lesions, both basal and non-basal cells play a role. Furthermore, all-trans retinol inhibited CSC-induced basal cell proliferation. Chemicals/CAS: retinol, 68-26-8, 82445-97-4; Dimethyl Sulfoxide, 67-68-5; Vitamin A, 11103-57-4