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Matrix metalloproteinases-3, -8, -9 as markers of disease activity and joint damage progression in early rheumatoid arthritis

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Author: Tchetverikov, I. · Lard, L.R. · Groot, J. de · Verzijl, N. · TeKoppele, J.M. · Breedveld, F.C. · Huizinga, T.W.J. · Hanemaaijer, R.
Institution: TNO Preventie en Gezondheid
Source:Annals of the Rheumatic Diseases, 11, 62, 1094-1099
Identifier: 237374
doi: doi10.1136/ard.62.11.1094
Keywords: Biology · Biomedical Research · gelatinase B · neutrophil collagenase · stromelysin · aged · arthropathy · disease activity · disease course · disease marker · enzyme activity · enzyme blood level · enzyme linked immunosorbent assay · female · human · joint destruction · longitudinal study · major clinical study · male · prediction · rheumatoid arthritis · Adult · alpha-Macroglobulins · Arthritis, Rheumatoid · Biological Markers · C-Reactive Protein · Disease Progression · Female · Humans · Joints · Male · Matrix Metalloproteinase 3 · Matrix Metalloproteinase 9 · Matrix Metalloproteinases · Middle Aged · Prospective Studies · Statistics, Nonparametric


Objective: To analyse the relation between systemic levels of pro-MMP-3, -8, and -9 matrix metalloproteinase (MMP) activity in α2 macroglobulin (α2M)/MMP complexes and the progression of joint destruction in patients with recent onset rheumatoid arthritis (RA). Methods: 109 patients with RA of recent onset were entered into this longitudinal study. Patients were followed up for two years; clinical data, blood samples, and radiographs were obtained at baseline and at 1 and 2 years. Serum levels of MMPs were measured by sandwich ELISA and MMP activity assays. Results: During the two years joint damage progressed from 0 to 10 (median Sharp score, p<0.001). Stable levels of pro-MMP-3 and a significant decrease in the levels of pro-MMP-8 and -9 and α2M/MMP complexes were seen throughout the two years. Regression analysis showed that serum pro-MMP-3 levels at disease onset were independently associated with the progression of joint damage (B = 0.7, 95% CI 0.3 to 1.1, p = 0.001). Based on the rate of joint destruction, patients were divided into two subgroups: patients with mild and severe joint damage progression. The pro-MMP-3 levels were significantly higher in the group with severe compared with mild disease at all times. Levels of pro-MMP-8 and -9 were decreased in both groups, whereas α 2M/MMP complex levels decreased in the group with mild disease only. Conclusion: Serum levels of the MMPs studied are associated with disease activity, but serum pro-MMP-3 levels at the onset of disease are also predictive of joint damage progression. Chemicals/CAS: gelatinase B, 146480-36-6; stromelysin, 79955-99-0; alpha-Macroglobulins; Biological Markers; C-Reactive Protein, 9007-41-4; Matrix Metalloproteinase 3, EC; Matrix Metalloproteinase 9, EC; Matrix Metalloproteinases, EC 3.4.24.