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A dietary mixture containing fish oil, resveratrol, lycopene, catechins, and vitamins E and C reduces atherosclerosis in transgenic mice

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Author: Verschuren, L. · Wielinga, P.Y. · Duyvenvoorde, W. van · Tijani, S. · Toet, K. · Ommen, B. van · Kooistra, T. · Kleemann, R.
Source:Journal of Nutrition, 5, 141, 863-869
Identifier: 429731
doi: doi:10.3945/jn.110.133751
Keywords: Nutrition · alpha tocopherol · apolipoprotein E3 · ascorbic acid · biological marker · C reactive protein · catechin · cell adhesion molecule · cholesterol · cytokine · docosahexaenoic acid · endothelial leukocyte adhesion molecule 1 · epigallocatechin gallate · fibrinogen · fish oil · green tea extract · icosapentaenoic acid · intercellular adhesion molecule 1 · interleukin 1beta · lipid · lycopene · omega 3 700 · placebo · polyunsaturated fatty acid · resveratrol · serum amyloid A · triacylglycerol · unclassified drug · animal experiment · animal model · antiinflammatory dietary mixture · article · atherogenic diet · atherosclerosis · cholesterol blood level · controlled study · diet supplementation · dietary intake · drug efficacy · drug megadose · feeding · female · inflammation · long term care · male · mouse · nonhuman · powder · protein blood level · protein expression · risk factor · risk reduction · transgenic animal · treatment duration · treatment outcome · triacylglycerol blood level · vasculitis · omega 3 700 · Solgar Vitamin and Herb · Life · MSB - Microbiology and Systems Biology MHR - Metabolic Health Research · EELS - Earth, Environmental and Life Sciences


Chronic inflammation and proatherogenic lipids are important risk factors of cardiovascular disease (CVD). Specific dietary constituents such as polyphenols and fish oils may improve cardiovascular risk factors and may have a beneficial effect on disease outcomes. We hypothesized that the intake of an antiinflammatory dietary mixture (AIDM) containing resveratrol, lycopene, catechin, vitamins E and C, and fish oil would reduce inflammatory risk factors, proatherogenic lipids, and endpoint atherosclerosis. AIDM was evaluated in an inflammation model, male human C-reactive protein (CRP) transgenic mice, and an atherosclerosis model, female ApoE*3Leiden transgenic mice. Two groups of male human-CRP transgenic mice were fed AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 6 wk. The effects of AIDM on basal and IL-1ß-stimulated CRP expression were investigated. AIDM reduced cytokine-induced human CRP and fibrinogen expression in human-CRP transgenic mice. In the atherosclerosis study, 2 groups of female ApoE*3Leiden transgenic mice were fed an atherogenic diet supplemented with AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 16 wk. AIDM strongly reduced plasma cholesterol, TG, and serum amyloid A concentrations compared with placebo. Importantly, long-term treatment of ApoE*3Leiden mice with AIDM markedly reduced the development of atherosclerosis by 96% compared with placebo. The effect on atherosclerosis was paralleled by a reduced expression of the vascular inflammation markers and adhesion molecules inter-cellular adhesion molecule-1 and E-selectin. Dietary supplementation of AIDM improves lipid and inflammatory risk factors of CVD and strongly reduces atherosclerotic lesion development in female transgenic mice. © 2011 American Society for Nutrition.