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Effects of consumption of Brussels sprouts on plasma and urinary glutathione S-transferase class-α and -π in humans

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Author: Nijhoff, W.A. · Mulder, T.P.J. · Verhagen, H. · Poppel, G. van · Peters, W.H.M.
Type:article
Date:1995
Source:Carcinogenesis, 4, 16, 955-957
Identifier: 232966
Keywords: glucosinolate · glutathione transferase · adult · article · Brassica · controlled study · diet · enzyme blood level · enzyme linked immunosorbent assay · female · human · male · priority journal · sex difference · urine level · vegetable · Adult · Biological Markers · Brassica · Cross-Over Studies · Diet · Enzyme Induction · Female · Glucosinolates · Glutathione Transferase · Human · Isoenzymes · Male · Sex Factors · Vegetables

Abstract

The effects of consumption of glucosinolate-containing Brussels sprouts on plasma and urinary glutathione S-transferase (GST) class-α and -π were investigated. Five male and five female non-smoking volunteers were randomly assigned to two groups in a crossover design. Five persons started on a glucosinolate-free diet (control period), while the other five consumed 300 g of cooked Brussels sprouts per day, at the expense of 300 g of glucosinolate-free vegetables (sprouts period). Dietary regimes were reversed after 1 week, GST levels were measured by enzyme-linked immunoabsorbent assay. At the end of the sprouts period, a significant increase (1.5-fold) in plasma class-α GST levels was observed in males but not in females (control versus sprouts, paired t-test; P-values 0.031 and 0.317 respectively), while plasma GST class-π levels as well as secretion of urinary GST class-α and -π levels remained unchanged. We conclude that (i) increased plasma GST class-α levels in males originate probably solely from the liver and not from stomach, intestine or kidney; (ii) males are more susceptible for induction of hepatic GSTs than females; and (iii) urinary GST concentration seems less useful as a biomarker for hepatic GST induction.