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Short term inhalation toxicity studies with peroxyacetyl nitrate in rats

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Author: Kruysse, A. · Feron, V.J. · Immel, H.R.
Institution: Centraal instituut voor voedingsonderzoek TNO
Source:Toxicology, 2, 8, 231-249
Identifier: 228285
Keywords: hemoglobin · behavior · drug toxicity · erythrocyte count · growth retardation · hematocrit · histology · inhalation · inhalational drug administration · intoxication · lung edema · microscopy · respiratory tract infection · theoretical study · Acetic Acids · Air Pollutants · Air Pollutants, Environmental · Animal · Body Weight · Comparative Study · Environmental Exposure · Female · Male · Nitrogen Oxides · Ozone · Peracetic Acid · Rats · Respiratory Tract Diseases · Time Factors · Medicine · Geneeskunde · Physiology · Fysiologie


The inhalation toxicity of peroxyacetyl nitrate (PAN) was examined in acute (single exposure), subacute (4-wk repeated exposure) and subchronic (13-wk repeated exposure) studies in rats. The 4-hr LC50 was found to be 95 ppm. In the 4-wk study rats were exposed to 0, 0.9, 4.1 or 11.8 ppm PAN vapor for 6 hr/day, 5 days/wk. Exposure to 11.8 ppm caused abnormal behavior, growth retardation, mortality, elevated hemoglobin contents, hematocrit values and erythrocyte counts, increased lung weights and severe inflammatory changes and epithelial hyper- and metaplasia in the respiratory tract. At 4.1 ppm minimal behavioral disturbance, transient growth depression, slightly increased lung weights and mild histopathologic changes in the respiratory tract were found. At 0.9 ppm no treatment-related alterations were detected. In the 13-wk study rats were exposed to 0, 0.2, 1.0 or 4.6 ppm PAN vapor for 6.5 hr/day, 5 days/wk. Exposure to 4.6 ppm resulted in changes similar to those found at 11.8 ppm in the 4-wk experiment, but no mortality occurred. At 1.0 ppm minimal irritation of the mucous membranes in the nasal cavity was the only PAN-related effect observed. No treatment-related changes were seen at 0.2 ppm. It was concluded that the no-toxic-effect level is between 0.2 and 1.0 ppm, and very probably close to the upper value. Chemicals/CAS: hemoglobin, 9008-02-0; Acetic Acids; Air Pollutants; Air Pollutants, Environmental; Nitrogen Oxides; Ozone, 10028-15-6; Peracetic Acid, 79-21-0