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Biosensors and multiple mycotoxin analysis

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Author: Gaag, B. van der · Spath, S. · Dietrich, H. · Stigter, E. · Boonzaaijer, G. · Osenbruggen, T. van · Koopal, K.
Type:article
Date:2003
Source:Food Control, 4, 14, 251-254
Identifier: 237104
doi: doi:10.1016/S0956-7135(03)00008-2
Keywords: Packaging · Analytical research · aflatoxin B1 · fumonisin B1 · mycotoxin · ochratoxin · toxin antibody · vomitoxin · zearalenone · article · bioassay · biosensor · concentration (parameters) · controlled study · cost benefit analysis · extraction · food analysis · immunochemistry · incubation time · instrumentation · measurement · sampling · surface plasmon resonance · toxin analysis

Abstract

An immunochemical biosensor assay for the detection of multiple mycotoxins in a sample is described.The inhibition assay is designed to measure four different mycotoxins in a single measurement, following extraction, sample clean-up and incubation with an appropriate cocktail of anti-mycotoxin antibodies.The different mycotoxins could be detected simultaneously in relevant concentrations within a time frame of 25 minutes, including the time needed for sensor regeneration.The application of the developed assay on presently available miniature SPR devices allows the development for low-cost instruments, which can be used in field measurements. © 2003 Elsevier Science Ltd. All rights reserved.