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Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis

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Author: Kajikawa, A. · Satoh, E. · Leer, R.J. · Yamamoto, S. · Igimi, S.
Type:article
Date:2007
Institution: TNO Kwaliteit van Leven
Source:Vaccine, 18, 25, 3599-3605
Identifier: 239976
doi: doi:10.1016/j.vaccine.2007.01.055
Keywords: Biology · Biotechnology · Flagellin · Lactobacillus · Salmonella · Vaccine · antibody · antigen · flagellin · gamma interferon · recombinant antigen · animal experiment · animal model · animal tissue · antibody specificity · antigen antibody complex · antigen expression · antigen specificity · article · bacterial infection · bacterial strain · cellular immunity · drug efficacy · ex vivo study · feces analysis · female · immune response · immunity · immunization · Lactobacillus casei · lymphocyte · mouse · nonhuman · priority journal · Salmonella enterica · Salmonella enteritidis · salmonellosis · serum · Administration, Oral · Animals · Female · Flagellin · Humans · Immunization · Interferon Type II · Lactobacillus casei · Lymphocyte Activation · Mice · Mice, Inbred C3H · Recombination, Genetic · Salmonella enteritidis · Salmonella Infections, Animal · Salmonella Vaccines

Abstract

A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-γ on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed. © 2007 Elsevier Ltd. All rights reserved.