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Brown Norway rat ovalbumin-specific immunoglobulin E antibodies increase the human basophil expression of CD63 marker

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Author: Bellou, A. · Saint-Laudy, J. · Knippels, L. · Montémont, C. · Vauthier, E. · Gerard, P. · Pellegrom, H. · Koerkamp, E.K. · Lesesve, J.F. · Guéant, J.L. · Lambert, H. · Mallié, J.P.
Type:article
Date:2003
Institution: TNO Voeding
Source:Scandinavian Journal of Immunology, 3, 57, 271-278
Identifier: 236989
doi: doi:10.1046/j.1365-3083.2003.01233.x
Keywords: Toxicology Biology · Toxicology and Applied Pharmacology · CD63 antigen · immunoglobulin E · ovalbumin · adolescent · allergy test · anaphylactic shock · animal model · antibody response · antibody specificity · antigen expression · article · basophil · cell activation · enzyme linked immunosorbent assay · histamine release · human · human cell · immunization · nonhuman · passive skin anaphylaxis · priority journal · rat · rat strain · sensitization · serum · Th2 cell · Anaphylaxis · Animals · Antigens, CD · Basophils · Enzyme-Linked Immunosorbent Assay · Histamine Release · Humans · Immunization · Immunoglobulin E · Ovalbumin · Passive Cutaneous Anaphylaxis · Platelet Membrane Glycoproteins · Rats · Rats, Inbred BN

Abstract

Anaphylactic shock is an immunoglobulin E (IgE)-dependent hypersensitivity. Biological tests like leucocyte histamine release (LHR) and human basophil activation (HBA), frequently used in human allergy, reflect both the amount of IgE fixed on cells and the cellular reactivity. To assess whether serum-specific IgE from Brown Norway (BN) rats prepared for ovalbumin (OVA)-induced anaphylactic shocks can activate human basophils which has a potential interest in experimental allergy: such a test could rapidly assert an IgE sensitization in laboratory animals genetically T-helper 2 (Th2)-predisposed. Rats (n = 39) were immunized three times (day 0, day 5 and day 21) with OVA injected subcutaneously. One week after the third immunization, a shock was induced with an intravenous (i.v.) bolus of OVA. Sensitization was assessed by passive cutaneous anaphylaxis (PCA) test and dosages of serum IgE antibodies anti-OVA by enzyme-linked immunosorbent assay. Blood basophils were counted before and during the shock. Before the shock induction (at day 21), an LHR test was performed on rat blood, and human basophils were sensitized with rat sera. HBA was demonstrated by the increase in the percentage of cells expressing CD63 antigen membrane, measured by flow cytometry. Twenty-one days after the first subcutaneous (s.c.) immunization, the rat serum induced a significant HBA. HBA was observed neither with the same serum previously heated nor with the serum from nonimmunized rats (NIRs). OVA-specific IgEs were significantly increased in immunized rat (IR) serum. The PCA test was negative when the serum was previously heated (56°C). We never observed any circulating basophils, and LHR test was negative. After OVA i.v. administration, all IRs died rapidly. HBA testing strongly suggests a mediation by specific IgE in the increase of CD63 in BN rats. Thus, HBA test seems useful in assessing whether an experimental allergy was induced in animals genetically predisposed to an immune response, Th2-mediated, like BN rat. We also conclude that rat basophil activation does not participate in the histamine release during anaphylactic shock in sensitized BN rats.