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Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region and in a low-energy collision cell

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Author: Dongen, W.D. van · Wijk, J.I.T. van · Green, B.N. · Heerma, W. · Haverkamp, J.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Rapid Communications in Mass Spectrometry, 17, 13, 1712-1716
Identifier: 86930
Keywords: Nutrition · Peptide · Peptide fragment · Chemistry · Comparative study · Instrumentation · Mass spectrometry · Methodology · Mass Spectrometry · Peptide Fragments · Peptides


A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.Chemicals/CAS: Peptide Fragments; Peptides