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Local lymph node activation in rats after dermal application of the sensitizers 2,4-dinitrochlorobenzene and trimellitic anhydride

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Author: Arts, J.H.E. · Dröge, S.C.M. · Bloksma, N. · Kuper, C.F.
Institution: Instituut CIVO-Toxicologie en Voeding TNO
Source:Food and Chemical Toxicology, 1, 34, 55-62
Identifier: 68136
doi: doi:10.1016/0278-6915(95)00089-5
Keywords: Toxicology · Administration, Topical · Allergens · Animals · Antimetabolites · Bromodeoxyuridine · Cell Division · Dinitrochlorobenzene · Dose-Response Relationship, Drug · Ear, External · Female · Irritants · Kidney · Liver · Lymph Nodes · Mice · Mice, Inbred BALB C · Organ Size · Phthalic Anhydrides · Rats · Rats, Inbred BN · Rats, Inbred F344 · Rats, Inbred Strains · Rats, Sprague-Dawley · Rats, Wistar · Skin · Spleen


Five rat strains were compared for their performance in the local lymph node assay (LLNA) a promising test system for the identification of the skin-sensitizing potential of chemicals in the mouse. The contact sensitizer 2,4-dinitrochlorobenzene (DNCB) and the contact and respiratory sensitizer trimellitic anhydride (TMA) were used as model chemicals and responses in rats were compared with those in BALB/c mice. The chemicals were applied to the dorsum of both ears, once daily for three consecutive days; 2 days (mice) or 3 days (rats) thereafter, proliferating cells were labelled by ip injection of BrdU 2 hr before the animals were killed. Systemic effects were subsequently assessed by determination of spleen liver and kidney weights, skin effects by determination of swelling and inflammatory cell infiltration of the ears, and immune effects by determination of weight and proliferative activity of the local lymph nodes (LLN). Following application ( x 3) of DNCB or TMA, minor systemic effects were observed, as indicated by slightly elevated spleen and liver weights in a few rat strains and the mice. Skin effects, consisting of increased ear thickness and presence of mononuclear inflammatory cell infiltrates, were observed in all rat strains treated with DNCB or TMA. LLN weights had increased, as had the proliferative activity in these nodes. It was concluded that effects induced by DNCB and TMA in all five rat strains were comparable with those in mice. Chemicals/CAS: Allergens; Antimetabolites; Bromodeoxyuridine, 59-14-3; Dinitrochlorobenzene, 97-00-7; Irritants; Phthalic Anhydrides; trimellitic anhydride, 552-30-7