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Retrospective detection of exposure to sulfur mustard: Improvements on an assay for liquid chromatography-tandem mass spectrometry analysis of albumin/sulfur mustard adducts

Author: Noort, D. · Fidder, A. · Hulst, A.G. · Woolfitt, A.R. · Ash, D. · Barr, J.R.
Type:article
Date:2004
Institution: Prins Maurits Laboratorium TNO
Source:Journal of Analytical Toxicology, 5, 28, 333-338
Identifier: 237891
Keywords: Toxicology · Tripeptide · Alkylation · Autoanalysis · Blood analysis · Chemical analysis · Chemical warfare · Controlled study · Digestion · Human · Human tissue · Liquid chromatography · Mass spectrometry · Protein isolation · Serum · Standard · Albumins · Alkylation · Chemical warfare agents · Chromatography, Affinity · Environmental exposure · Humans · Reference values · Retrospective studies · Mass spectrometry · Electrospray ionization · Mustard gas, 505-60-2 · Pronase, 9036-06-0 · Albumins

Abstract

We here report on the further development of the method comprising the pronase digestion of albumin alkylated by sulfur mustard and the subsequent mass spectrometric analysis of an adducted tripeptide. This includes significant improvements in both the albumin isolation procedure and the automation of the microliquid chromatography-electrospray-tandem mass spectrometric analysis. We also report on the results of a small reference range study, in which we have established that there are no detectable interferences in sera from unexposed individuals.