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Lysine-based cluster mannosides that inhibit ligand binding to the human mannose receptor at nanomolar concentration

Author: Biessen, E.A.L. · Noorman, F. · Teijlingen, M.E. van · Kuiper, J. · Barrett-Bergshoeff, M.M. · Bijsterbosch, M.K. · Rijken, D.G. · Berkel, T.J.G. van
Type:article
Date:1996
Institution: Gaubius Instituut TNO
Source:Journal of Biological Chemistry, 45, 271, 28024-28030
Identifier: 233558
doi: doi:10.1074/jbc.271.45.28024
Keywords: Biology · Mannose receptor · Mannoside · Binding site · Ligand binding · Receptor affinity · receptor binding · Binding, Competitive · Biotin · Chromatography, Thin Layer · Humans · Kinetics · Lectins · Lectins, C-Type · Lysine · Magnetic Resonance Spectroscopy · Mannose-Binding Lectins · Oligosaccharides · Receptors, Cell Surface · Ribonucleases · Tissue Plasminogen Activator

Abstract

In search of synthetic high affinity ligands for the mannose receptor, we synthesized a series of lysine-based oligomannosides containing two (M2L) to six (M6L5) terminal α-D-mannose groups that are connected with the backbone by flexible elongated spacers (16 Å). The synthesized cluster mannosides were all able to displace binding of biotinylated ribonuclease B and tissue-type plasminogen activator to isolated human mannose receptor. The affinity of these cluster mannosides for the mannose receptor was continuously enhanced from 18-23 μM to 0.5-2.6 nM, with mannose valencies increasing from two to six. On average, expansion of the cluster mannoside with an additional α-D-mannose group resulted in a 10-fold increase in its affinity for the mannose receptor. M3L2 to M6L5 displayed negative cooperative inhibition of ligand binding to the mannose receptor, suggesting that binding of these mannosides involves multiple binding sites. The nanomolar affinity of the most potent ligand, the hexamannoside M6L5 makes it the most potent synthetic cluster mannoside for the mannose receptor yet developed. As a result of its high affinity and accessible synthesis, M6L5 not only is a powerful tool to study the mechanism of ligand binding by the mannose receptor, but it is also a promising targeting device to accomplish cell-specific delivery of genes and drugs to liver endothelial cells or macrophages in bone marrow, lungs, spleen, and atherosclerotic plaques. Chemicals/CAS: Biotin, 58-85-5; Lectins; Lectins, C-Type; Lysine, 56-87-1; mannose receptor; Mannose-Binding Lectins; oligomannoside; Oligosaccharides; Receptors, Cell Surface; ribonuclease B, EC 3.1.27.-; Ribonucleases, EC 3.1.-; Tissue Plasminogen Activator, EC 3.4.21.68