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Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

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Author: Martínez, B. · Sillanpää, J. · Smit, E. · Korhonen, T.K. · Pouwels, P.H.
Type:article
Date:2000
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Bacteriology, 23, 182, 6857-6861
Identifier: 41891
doi: doi:10.1128/JB.182.23.6857-6861.2000
Keywords: Nutrition · Bacterial protein · Binding protein · Collagen binding s protein · Unclassified drug · Bacterium adherence · Gene expression · Lactobacillus · Lactobacillus casei · Lactobacillus crispatus · Nonhuman · Priority journal · Protein secretion · Signal transduction · Bacterial Proteins · Cell Membrane · Integrins · Lactobacillus · Lactobacillus casei · Membrane Glycoproteins · Membrane Proteins · Receptors, Collagen · Bacteria (microorganisms) · Lactobacillus casei · Lactobacillus crispatus · Posibacteria

Abstract

The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens. Chemicals/CAS: Bacterial Proteins; Integrins; Membrane Glycoproteins; Membrane Proteins; Receptors, Collagen; surface array protein, bacteria