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Mannosylated PLP139-151 induces peptide-specific tolerance to experimental autoimmune encephalomyelitis

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Author: Luca, M.E. · Kel, J.M. · Rijs, W. van · Drijfhout, J.W. · Koning, F. · Nagelkerken, L.
Institution: Gaubius Instituut TNO
Source:Journal of Neuroimmunology, 1-2, 160, 178-187
Identifier: 238383
doi: doi:10.1016/j.jneuroim.2004.11.014
Keywords: C-type lectins · EAE · Mannose-receptor · Tolerance · immunoglobulin G2 · myelin proteolipid protein[139-151] · proteolipid protein · unclassified drug · allergic encephalomyelitis · animal cell · animal experiment · animal model · animal tissue · cell migration · cell proliferation · controlled study · delayed hypersensitivity · drug effect · drug efficacy · female · immunization · mouse · nonhuman · priority journal · T lymphocyte · Amino Acid Sequence · Animals · Cell Movement · Cells, Cultured · Central Nervous System · Encephalomyelitis, Autoimmune, Experimental · Female · Hypersensitivity, Delayed · Immunodominant Epitopes · Injections, Subcutaneous · Leukocytes, Mononuclear · Mannose · Mice · Molecular Sequence Data · Myelin Proteolipid Protein · Peptide Fragments · Self Tolerance · Severity of Illness Index · Th1 Cells


SJL mice immunized with mannosylated (M-) PLP139-151 in complete adjuvant do not develop EAE and little CNS mononuclear cell infiltration; other mannosylated peptides were ineffective in this experimental setting. Despite apparently normal T cell responses, M-PLP139-151-immunized mice show impaired delayed-type-sensitivity to PLP139-151 but a normal response to other peptides. After re-immunization with PLP139-151 in complete adjuvant, these mice are largely tolerant to EAE, show less T cell proliferation and decreased peptide-specific IgG2a. Our data suggest that M-PLP139-151 induces peptide-specific tolerance to EAE via a mechanism of deletion or impaired migration of encephalitogenic T cells. © 2004 Elsevier B.V. All rights reserved. Chemicals / CAS: Immunodominant Epitopes; Mannose, 31103-86-3; Myelin Proteolipid Protein; Peptide Fragments; proteolipid protein 139-151