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Increase in the advanced glycation end product pentosidine in Bruch's membrane with age

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Author: Handa, J.T. · Verzijl, N. · Matsunaga, H. · Aotaki-Keen, A. · Lutty, G.A. · Koppele, J.M. te · Miyata, T. · Hjelmeland, L.M.
Source:Investigative Ophthalmology and Visual Science, 3, 40, 775-779
Identifier: 234977
Keywords: Adult · Aged · Aged, 80 and over · Aging · Arginine · Bruch Membrane · Choroid · Chromatography, High Pressure Liquid · Diabetic Retinopathy · Glycosylation End Products, Advanced · Humans · Immunoenzyme Techniques · Infant · Lysine · Middle Aged · Pigment Epithelium of Eye · Retinal Drusen


PURPOSE. To determine whether there is an age-related increase of pentosidine in human Bruch's membranes and to localize pentosidine and carboxymethyllysine (CML), two well-characterized, advanced glycation end products (AGEs) in aged human Bruch's membranes and choroid in vivo. METHODS. Human Bruch's membrane samples were isolated from the retinal pigment epithelium (RPE) and choroid and subjected to reversed-phase high-performance liquid chromatography to determine pentosidine content. A polyclonal anti- pentosidine antibody and a monoclonal antibody specific for carboxymethyllysine were used to localize AGEs in 20-month-old nondiabetic, 82-year-old nondiabetic, and 82-year-old diabetic globes. RESULTS. Human Bruch's membranes (n = 20) showed a linear age-dependent increase in pentosidine that reached approximately 0.17 millimoles pentosidine per mole hydroxyproline in late life (r = 0.896; P < 0.001). Immunohistochemical evaluation showed evidence of pentosidine in Bruch's membrane, choroidal extracellular matrix, and vessel walls in the 82-year-old nondiabetic and diabetic globes. A similar staining pattern was found with the anti-CML antibody. Basal laminar deposits and drusen stained with both antibodies in the elderly nondiabetic eye. In contrast, neither antibody stained the 20- month-old tissue. CONCLUSIONS. We provide biochemical and immunohistochemical evidence for the formation of pentosidine and CML structures in human Bruch's membrane and choroid with age. These changes could promote aging of the RPE- Bruch's membrane-choroid complex.Chemicals/CAS: Arginine, 74-79-3; Glycosylation End Products, Advanced; Lysine, 56-87-1; N(6)-carboxymethyllysine, 5746-04-3; pentosidine, 124505-87-9