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Effect of propionyl-L-carnitine on human endothelial cells

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Author: Hinsbergh, V.W.M. van · Scheffer, M.A.
Institution: Gaubius Instituut TNO
Source:Cardiovascular Drugs and Therapy, SUPPL. 1, 5, 97-106
Identifier: 231442
Keywords: Biology · Acetylcarnitine · Calcium · Carnitine · Cells, Cultured · Endothelium, Vascular · Human · Lipoproteins, LDL


A possible protective effect of propionyl-L-carnitine on human endothelial cells was studied both under basal culture conditions and in the presence of agents capable of influencing oxidative damage, such as glucose/glucose oxidase and oxidized low-density lipoproteins. Propionyl-L-carnitine had no significant effect on the observed decline in various parameters of cell viability, e.g., cell detachment, release of lactate dehydrogenase, and the rate of protein synthesis. Propionyl-L-carnitine progressively decreased the fluorescence intensity in fura-2-loaded endothelial cells obtained during excitation at 340 nm. A similar effect was observed with propionyl-L-carnitine and acetyl-L-carnitine, but not with L-carnitine and D-carnitine. These results suggest that propionyl-L-carnitine and acetyl-L-carnitine decrease the cytoplasmic calcium level in endothelial cells.