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Steviol Glycoside Rebaudioside A Induces Glucagon-like Peptide-1 and Peptide YY Release in a Porcine ex Vivo Intestinal Model

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Author: Ripken, D. · Wielen, N. van der · Wortelboer, H.M. · Meijerink, J. · Witkamp, R.F. · Hendriks, H.F.J.
Type:article
Date:2014
Source:Journal of Agricultural and Food Chemistry, 33, 62, 8365-8370
Identifier: 510985
doi: doi:10.1021/jf501105w
Keywords: Nutrition · Rebaudioside A · GLP-1 · PYY · Ex vivo intestinal porcine model · Casein · Peptides · Sugar (sucrose) · Glucagon-like peptide-1 · Porcine models · PYY · Small intestine · Steviol glycosides · Weight management · Tissue · Food and Nutrition · Healthy Living · Life · MSB - Microbiology and Systems Biology · ELSS - Earth, Life and Social Sciences

Abstract

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called “ileal brake”. The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm2 of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm2 of tissue, p < 0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm2 of tissue, p < 0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm2 of tissue, p < 0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm2 of tissue, p < 0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm2 of tissue, p < 0.01). These findings may help in developing dietary approaches for weight management.