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Carbon monoxide and cyanide as intrinsic ligands to iron in the active site of [NiFe]-hydrogenases. NiFe(CN)2CO, biology's way to activate H2

Author: Pierik, A.J. · Roseboom, W. · Happe, R.P. · Bagley, K.A. · Albracht, S.P.J.
Type:article
Date:1999
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Biological Chemistry, 6, 274, 3331-3337
Identifier: 234939
doi: doi:10.1074/jbc.274.6.3331
Keywords: Nutrition · Carbon monoxide · Cyanide · Hydrogenase · Chromatium vinosum · Complex formation · Enzyme active site · Enzyme subunit · Gene cluster · Hydrogen bond · Infrared spectroscopy · Iron metabolism · Ligand binding · Membrane binding · Nonhuman · Oxidation reduction reaction · Priority journal · Binding Sites · Carbon Isotopes · Chromatium · Cyanides · Electron Spin Resonance Spectroscopy · Hydrogen · Hydrogenase · Iron · Ligands · Nitrogen Isotopes · Spectroscopy, Fourier Transform Infrared · Allochromatium vinosum · Chromatium · Desulfovibrio gigas · Desulfovibrio vulgaris

Abstract

Infrared-spectroscopic studies on the [NiFe]-hydrogenase of Chromatium vinosum-enriched in 15N or 13C, as well as chemical analyses, show that this enzyme contains three non-exchangeable, intrinsic, diatomic molecules as ligands to the active site, one carbon monoxide molecule and two cyanide groups. The results form an explanation for the three non-protein ligands to iron detected in the crystal structure of the Desulfovibrio gigas hydrogenase (Volbeda, A., Garcin, E., Piras, C., De Lacey, A. I., Fernandez, V. M., Hatchikian, E. C., Frey, M., and Fontecilla-Camps, J. C. (1996) J. Am. Chem. Soc. 118, 12989-12996) and for the low spin character of the lone ferrous iron ion observed with Mossbauer spectroscopy (Surerus, K. K., Chen, M., Van der Zwaan, W., Rusnak, F. M., Kolk, M., Duin, E. C., Albracht, S. P. J., and Munck, E. (1994) Biochemistry 33, 4980-4993). The results do not support the notion, based upon studies of Desulfovibrio vulgaris [NiFe]-hydrogenase (Higuchi, Y., Yagi, T., and Noritake, Y. (1997) Structure 5, 1671-1680), that SO is a ligand to the active site. The occurrence of both cyanide and carbon monoxide as intrinsic constituents of a prosthetic group is unprecedented in biology.