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Effect of chronic graft-versus-host disease on the intestine in adult BDF1 mice

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Author: Geus, B. de · Hogenesch, H. · Heer, E. de · Bruijn, J.A. · Enden, M. van den · Rozing, J.
Type:article
Date:1993
Institution: Instituut voor Verouderings- en Vaatziekten Onderzoek TNO
Source:International Journal of Experimental Pathology, 4, 74, 371-377
Identifier: 232086
Keywords: Biology · Animal model · Chronic graft-versus-host disease · Intestine · Intraepithelial lymphocytes · Animal model · Animal tissue · Controlled study · Enteropathy · Graft versus host reaction · Histology · Lymphocyte · Mouse · Nonhuman · Animal · Antigens, CD3 · Cells, Cultured · Chronic Disease · Disease Models, Animal · Graft vs Host Disease · Intestines · Lymphocytes · Mice · Mice, Inbred C57BL · Mice, Inbred DBA

Abstract

This study was performed to characterize the intestinal lesions in chronic graft-versus-host disease (GVHD) in mice and to determine a possible role of intestinal intraepithelial lymphocytes (iIEL) in the development of these lesions. Chronic GVHD was induced by transfer of DBA/2 lymphocytes into non-irradiated (C57BL/10 x DBA/2)F1 (BDF1) recipients. There was mild to moderate mucosal oedema with multifocal mixed inflammatory cell infiltrations in the small intestine. The caecum was more severely affected with severe oedema, progressive loss of crypts and severe distortion of the mucosal architecture. The total number of iIEL did not change during the development of chronic GVHD, but there were alterations in the composition of the iIEL population. An increase of CD3+, Thy-1+ cells was accompanied by an increase of TCRαβ+ cells and a decrease of TCRγδ+ cells. There was no evidence of infiltration of donor lymphocytes into the intestinal epithelium as determined by the H2K haplotype of the iIEL. These lesions differ from previously described models of chronic GVHD, induced by DBA/2 donor lymphocytes in BDF1 recipients. We suggest that the haemopoietic organs that are used as the source of donor lymphocytes determine the outcome of the GVHD. Modulation of the composition of the donor lymphocyte population may be useful in the establishment of relevant animal models of human enteropathy. Chemicals/CAS: Antigens, CD3