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Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced

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Author: Hijum, S.A.F.T. · Bonting, K. · Maarel, M.J.E.V. van der · Dijkhuizen, L.
Type:article
Date:2001
Institution: TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO
Source:FEMS Microbiology Letters, 205, 323-328
Identifier: 72488
Keywords: Biology · Amino Acid Sequence · Fructans · Hexosyltransferases · Lactobacillus · Magnetic Resonance Spectroscopy · Molecular Sequence Data · Molecular Weight · Polysaccharides, Bacterial · Sequence Alignment · Sucrose · Lactobacillus · Lactobacillus reuteri · Lactobacillus sp. · Posibacteria

Abstract

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells. © 2001 Pubblished by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies. Chemicals/CAS: Fructans; Hexosyltransferases, EC 2.4.1.-; levan fructotransferase, EC 2.4.1.-; levan, 9013-95-0; levansucrase, EC 2.4.1.10; Polysaccharides, Bacterial; Sucrose, 57-50-1