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Application of liquid chromatography-mass spectrometry to measure the concentrations and study the synthesis of short chain fatty acids following stable isotope infusions

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Author: Meesters, R.J.W. · Eijk, H.M.H. van · Have, G.A.M. ten · Graaf, A.A. de · Venema, K. · Rossum, B.E.J. van · Deutz, N.E.P.
Type:article
Date:2007
Institution: TNO Kwaliteit van Leven
Source:Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1-2, 854, 57-62
Identifier: 240040
doi: doi:10.1016/j.jchromb.2007.03.044
Keywords: Health · Biomedical Research · LC-MS · SCFA · Short chain fatty acids · Stable isotope · Concentration (process) · Measurement theory · Synthesis (chemical) · Short chain fatty acids · Stable isotope · Liquid chromatography · acetic acid · butyric acid · propionic acid · short chain fatty acid · tracer · animal experiment · colon · fatty acid synthesis · ion exclusion chromatography · mass spectrometry · nonhuman · priority journal · steady state · Animals · Chromatography, Gel · Fatty Acids · Isotopes · Mass Spectrometry · Swine · Suidae

Abstract

A new method involving zinc sulphate deproteinization was developed to study short chain fatty acids (SCFA) production in the colon and subsequent occurrence of SCFA in blood. SCFA were baseline separated in a 30 min cycle using ion-exclusion chromatography and detected by mass spectrometry. Concentrations could be measured down to 10 μM and isotopomeric distributions could be assessed, enabling the conduction of tracer studies to study changes in SCFA synthesis. The applicability of the method was tested in an extensively characterized pig model yielding portal SCFA concentrations ranging from 70 μM (butyric acid) to 150 μM (propionic acid) to 440 μM (acetic acid) prior to butyrate tracer infusion, reaching butyric acid isotopic steady state within 2 h. © 2007 Elsevier B.V. All rights reserved.