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Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique

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Author: Barbosa, M.J. · Zijffers, J.W. · Nisworo, A. · Vaes, W. · Schoonhoven, J. van · Wijffels, R.H.
Type:article
Date:2005
Institution: TNO Kwaliteit van Leven
Source:Biotechnology and Bioengineering, 2, 89, 233-242
Identifier: 238309
doi: doi:10.1002/bit.20346
Keywords: Health · Analytical research · A-stat · Carotenoids · Flat-panel · Light · Microalgae · Vitamins · Algae · Biomass · Cell culture · Optimization · Vitamins · Carotenoid · Light intensities · Microalgae · Photobioreactors · Bioreactors · carotenoid · vitamin · alga · article · biomass · chemostat · light · light intensity · nonhuman · photobioreactor · reaction optimization · reactor · Algae, Green · Ascorbic Acid · Bioreactors · Carotenoids · Cell Culture Techniques · Cell Proliferation · Computer Simulation · Energy Transfer · Flow Injection Analysis · Gene Expression Regulation, Bacterial · Light · Models, Biological · Vitamin E

Abstract

Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and product yield of continuous cultures of the microalgae D. tertiolecta in a flat-panel reactor. In this study, four different accelerations were studied, a pseudo steady state was maintained at acceleration rates of 0.00016 and 0.00029 h-2 and results were similar to those of the chemostat. An increase in the acceleration rate led to an increase in the deviation between results obtained in the A-stat and in the chemostats. We concluded that it is advantageous to use the A-stat instead of chemostats to determine culture characteristics and optimize a specific photobioreactor. The effect of average light intensity inside the photobioreactor on the production of vitamins C and E, lutein, and β-carotene was studied using the A-stat. The highest concentrations of these products were 3.48 ± 0.46, 0.33 ± 0.06, 5.65 ± 0.24, and 2.36 ± 0.38 mg g-1 respectively. These results were obtained at different average light intensities, showing the importance of optimizing each product on light intensity. © 2004 Wiley Periodicals, Inc.