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Retrospective detection of sulfur mustard exposure by mass spectrometric analysis of adducts to albumin and hemoglobin: An in vivo study

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Author: Noort, D. · Fidder, A. · Degenhardt-Langelaan, C.E.A.M. · Hulst, A.G.
Type:article
Date:2008
Institution: TNO Defensie en Veiligheid
Source:Journal of Analytical Toxicology, 1, 32, 25-30
Identifier: 240608
Keywords: Albumin · Alkylation · Animal experiment · Article · Blood analysis · Cell membrane · Chemical reaction · Controlled study · Environmental exposure · Erythrocyte · In vivo study · Nonhuman · Alkylation · Animals · Affinity chromatography · Liquid chromatography · Environmental exposure · Environmental monitoring · Injections, Intravenous · Male · Pronase · Rats · Wistar · Mass spectrometry · Electrospray ionization · Tandem mass spectrometry · Rattus · Hemoglobin 9008-02-0 · Mustard gas 505-60-2 · Biological markers · Hemoglobins · Pronase, EC 3.4.24.- · Serum albumin

Abstract

The persistence in rats of sulfur mustard adducts to albumin and hemoglobin was studied in vivo after exposure (intravenously; 0.3 mg/kg; approximately 0.1 LD50) of rats to sulfur mustard. The albumin adduct (S-HETE)Cys-Pro-Tyr was detectable up to 7 days after the exposure, while the adduct to the N-terminal valine in hemoglobin was still detected after 28 days. The decrease in adduct levels corresponded well with the half-life time of albumin in rats and with the lifetime of the rat erythrocyte. Remarkably, the N-terminal valine adduct to hemoglobin increased during the first three days, which implies that there is still free sulfur mustard present during that time. In contrast, the corresponding albumin adduct levels did not increase during this time period. The free sulfur mustard might have accumulated in the erythrocyte cell membrane.