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Food allergen (peanut)-specific TH2 clones generated from the peripheral blood of a patient with peanut allergy

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Author: Jong, E.C. de · Spanhaak, S. · Martens, B.P.M. · Kapsenberg, M.L. · Penninks, A.H. · Wierenga, E.A.
Type:article
Date:1996
Institution: TNO Voeding
Source:Journal of Allergy and Clinical Immunology, 1, 98, 73-81
Identifier: 233589
Keywords: Toxicology · Food allergy · Peanut · Phenotype · T-cell clones · Food allergen · Immunoglobulin e · Interleukin 4 · Interleukin 5 · Adolescent · Case report · Cell clone · Controlled study · Helper cell · T lymphocyte subpopulation · Adult · Antigens, Surface · Arachis hypogaea · Clone Cells · Cytokines · Epitopes · Female · Food Hypersensitivity · Humans · Lymphocyte Activation · Male · Plant Proteins · Th2 Cells

Abstract

Background: Increasing evidence indicates a prominent role of allergen-specific TH2 cells, with high IL-4 and IL-5 production and low interferon-γ production, in the regulation of IgE and eosinophil production in allergic disorders. However, most studies have concentrated on T cells reactive with inhalation allergens, whereas little is known about the properties of food allergen-reactive T cells. Objective: In this study we therefore characterized peanut-specific T cells, cloned from a patient with severe peanut allergy. Methods: Peripheral blood mononudear cells from patients with peanut allergy and nonallergic individuals were stimulated with crude peanut extract (CPE) to compare the proliferative responses and to select a suitable patient for the cloning of CPE-specific T cells. The resultant panel of CPE-reactive T-lymphocyte clones was serologically phenotyped by flow cytometty and analyzed for cytokine secretion by ELISA. Results: The patients' peripheral blood mononudear cells showed a dose-dependent proliferative response to CPE, which was significantly higher (p < 0.05) than in peripheral blood mononudear cells of nonallergic donors. The CPE-specific T-lymphocyte clones generated from the selected patient were all CD4+/CD8- T helper cells with a TH2 cytokine profile, secreting high amounts of IL-4 and IL-5, but little or no interferon-γ. Conclusions: This study demonstrates that peanut-specific T cells do occur in the peripheral blood of patients with peanut allergy and suggests an increased frequency of these T cells in patients compared with nonallergic control subjects. The CD4+ phenotype and the TH2 cytokine profile of the CPE-specific T-lymphocyte clones suggest a functional role of allergen-specific TH2 cells in the pathophysiology of food allergy, similar to the function of inhalation allergen-specific TH2 cells. Copyright © 1996 by Mosby-Year Book, Inc.