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Trophic support by neural explants of cultured muscle fibers

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Author: Hooisma, J. · Slaaf, D.W. · Meeter, E. · Stevens, W.F.
Type:article
Date:1978
Institution: Medisch Biologisch Laboratorium TNO
Source:Experimental Neurology, 3, 62, 628-646
Identifier: 228419
Keywords: Biology · Acetylcholine · Tubocurarine chloride · Animal experiment · Autonomic nervous system · Brain cortex · Cell culture · Central nervous system · Chicken · In vitro study · Mouse · Muscle · Muscle cell · Nerve cell · Nervous system · Neuromuscular synapse · Peripheral nervous system · Short survey · Spinal cord · Spinal ganglion · Sympathetic ganglion · Animal · Cell Differentiation · Cell Survival · Cells, Cultured · Cerebral Cortex · Chick Embryo · Ciliary Body · Comparative Study · Ganglia, Autonomic · Ganglia, Spinal · Membrane Potentials · Mice · Motor Endplate · Muscle Contraction · Muscles · Spinal Cord

Abstract

The influence of various neural explants on the morphology and the survival of chick muscle fibers was studied. A method was developed to evaluate the condition of the muscle fibers using the following four morphological parameters: cross striation, thickness, number of fibers and absence of vacuoles. Chick as well as mouse spinal cord explants appeared to have a distinct favorable influence on the muscle fibers. Chick ciliary ganglia and mouse cortex explants had less effect and chick sympathetic ganglia and mouse dorsal root ganglia had no effect. Innervation by spinal cord neurons did not lead to a change in resting membrane potential of the muscle fibers. The amount of cross striation in muscle fibers in the vicinity of mouse spinal cord explants was positively correlated with the frequency of spontaneous end-plate potentials in the muscle fibers. d-Tubocurarine did not interfere with the trophic support of muscle cells by nerve cells, although it reversibly blocked neuromuscular transmission throughout the experiment. This demonstrates that neither the acetylcholine receptor nor the activity induced in the muscle fiber mediate the trophic action. The data suggest that a humoral trophic factor, released at the neuromuscular junction or at a region of close cell-cell contact, is needed for normal development and maintenance of muscle fiber morphology. Chemicals/CAS: acetylcholine, 51-84-3, 60-31-1, 66-23-9; tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7