Repository hosted by TU Delft Library

Home · Contact · About · Disclaimer ·
 

The expression of histocompatibility-2 antigens on hemopoietic stem cells

Publication files not online:

Author: Russell, J.L. · Engh, G.J. van den
Type:article
Date:1979
Institution: Radiobiologisch Instituut TNO
Source:Tissue Antigens, 1, 13, 45-52
Identifier: 228639
Keywords: Animal · Cell Division · Cell Survival · Clone Cells · Colony-Forming Units Assay · Epitopes · H-2 Antigens · Hematopoietic Stem Cells · Immune Sera · Mice · Mice, Inbred C57BL · Mice, Inbred CBA · Papain · Spleen

Abstract

Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. Of antisera which are specific for subregions of the H-2 complex, only those which contain anti-H-2K or anti-H-2D activity are effective. Specific anti-Ia sera do not react with the stem cell. If the titer of the CFU-s suppressive effect of the antisera is compared to their toxicity to spleen lymphocytes, it can be concluded that CFU-s express antigens in the same density as spleen lymphocytes. The amount of H-2 antigens expressed on the surface of CFU-s is independent of its rate of proliferation. Chemicals/CAS: Epitopes; H-2 Antigens; Immune Sera; Papain, EC 3.4.22.2