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Radiosensitization of biologically active DNA in cellular extracts by oxygen. Evidence that the presence of SH compounds is not required

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Author: Hemmen, J.J. van · Meuling, W.J.A. · Bleichrodt, J.F.
Type:article
Date:1974
Institution: Medisch Biologisch Laboratorium TNO
Source:International Journal of Radiation Biology, 6, 26, 547-555
Identifier: 227740
Keywords: Biology · Bacteriophage dna · DNA · Oxygen · Radioisotope · Thiol derivative · Gamma radiation · In vitro study · Methodology · Microorganism · Radiosensitivity · Radiosensitization · Theoretical study · Azo Compounds · Cobalt Radioisotopes · Coliphages · Cyclic N-Oxides · Cysteamine · DNA Viruses · DNA, Circular · DNA, Viral · Dose-Response Relationship, Radiation · Escherichia coli · Ethylmaleimide · Gamma Rays · Mutation · Oxygen · Pseudomonas · Radiation Effects · Radiation-Sensitizing Agents · Sulfhydryl Compounds

Abstract

The radiosensitization by oxygen of biologically active bacteriophage DNA in bacterial extracts was studied. The oxygen effect in such a system appeared not to be due or only to a minor extent to the presence of endogenous sulphydryl compounds. The components in a cell extract which enable oxygen and other sensitizers to sensitize DNA could not be destroyed by extremely high doses of γ radiation. Chemicals/CAS: DNA, 9007-49-2; oxygen, 7782-44-7; thiol derivative, 13940-21-1; Azo Compounds; Cobalt Radioisotopes; Cyclic N-Oxides; Cysteamine, 60-23-1; DNA, Circular; DNA, Viral; Ethylmaleimide, 128-53-0; Oxygen, 7782-44-7; Radiation-Sensitizing Agents; Sulfhydryl Compounds