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Platinum compounds with anti-tumour activity

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Author: Plooy, A.C.M. · Lohman, P.H.M.
Type:article
Date:1980
Institution: Medisch Biologisch Laboratorium TNO
Source:Toxicology, 2, 17, 169-176
Identifier: 228885
doi: doi:10.1016/0300-483X(80)90090-6
Keywords: Biology · Antineoplastic agent · Cisplatin · Platinum derivative · Radioisotope · Animal experiment · Cytotoxicity · DNA damage · DNA strand breakage · DNA synthesis · Drug toxicity · Endocrine system · Female genital system · Heredity · In vitro study · Intoxication · Mutagenesis · Mutagenicity · Ovary cell · Structure activity relation · Thymidine h 3 · Alkalies · Animal · Antineoplastic Agents · DNA · DNA Replication · Hamsters · In Vitro · Platinum · Structure-Activity Relationship · Support, Non-U.S. Gov't

Abstract

Ten platinum (Pt) coordination complexes with different ligands, comprising both Pt(II) and Pt(IV) complexes of which the cis-compounds all possessed at least some anti-tumour activity and the trans-compounds were inactive, were tested as to their effect on cell survival and the induction and repair of DNA damage in cultured Chinese hamster cells (CHO). The tests used were the determination of cytotoxicity, inhibition of the semi-conservative DNA replication, induction of damage in DNA and mutagenicity. The Pt-compounds could be divided in 3 groups on the basis of their cytotoxicity. The compounds with high cytotoxicity were also active anti-tumour drugs, but a clear correlation between cytotoxicity and anti-tumour activity was not observed. All compounds inhibited semi-conservative DNA synthesis and induced DNA repair replication. No induction of DNA single strand breaks and DNA cross-links was detected. However, the occurrence of alkali labile sites in the DNA of treated CHO cells demonstrated the induction of unidentified DNA base damages. The 4 compounds tested so far, 2 cis- and 2 trans-Pt complexes, induced mutations in CHO cells, leading to resistance to 6-thioguanine; the cis-compounds were more mutagenic than the trans-compounds. Chemicals/CAS: cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; Alkalies; Antineoplastic Agents; DNA, 9007-49-2; Platinum, 7440-06-4