Repository hosted by TU Delft Library

Home · Contact · About · Disclaimer ·
 

New monoclonal antibodies against the putative immunosuppressive site of retroviral p15E

Publication files not online:

Author: Lang, M.S. · Oostendorp, R.A.J. · Simons, P.J. · Boersma, W. · Knegt, P. · Ewijk, W. van
Type:article
Date:1994
Source:Cancer Research, 7, 54, 1831-1836
Identifier: 280590
Keywords: Monoclonal antibody · Monoclonal antibody er is1 · Monoclonal antibody er is2 · Monoclonal antibody er is5 · Unclassified drug · Airus protein · Antibody production · Antigen antibody reaction · Antigen recognition · Cancer cell culture · Cancer immunotherapy · Head and neck cancer · Human · Human cell · Retrovirus · Squamous cell carcinoma · Virus infection · Amino Acid Sequence · Antibodies, Monoclonal · Binding Sites, Antibody · Blotting, Western · Carcinoma, Squamous Cell · Cell Line · Conserved Sequence · Enzyme-Linked Immunosorbent Assay · Epitopes · Head and Neck Neoplasms · Immunoblotting · Immunohistochemistry · Immunosuppression · Laryngeal Neoplasms · Lung Neoplasms · Lymphoma, Large-Cell · Molecular Sequence Data · Peptides · Rauscher Virus · Retroviridae · Retroviridae Proteins, Oncogenic · Tumor Cells, Cultured · Antibodies, Monoclonal · Binding Sites, Antibody · Epitopes

Abstract

Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral p15E. In order to study the relationship between SCC-HN associated immunosuppression and retroviral p15E, we produced three new monoclonal antibodies (MAbs: ER- IS1, ER-IS2, and ER-185) directed against the immunosuppressive synthetic CKS-17 peptide. These MAbs react with the immunosuppressive peptide (in enzyme-linked immunosorbent assay), with human tumor cell lines (in FACScan analysis), with retroviral p15E (on Western blot), and with cryostat sections of SCC-HN tumor tissue. In addition, the MAbs neutralize the immunosuppressive low molecular weight factors present in sera of patients with SCC-HN. These results show that retroviral p15E and the immunosuppressive factors associated with SCC-HN share a conserved immunosuppressive epitope and that MAbs against this epitope can be used for detection and centralization of the tumor-associated immunosuppressive protein(s).