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Evaluation of the antibacterial spectrum of drosocin analogues

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Author: Bikker, F.J. · Kaman-van Zanten, W.E. · Vries-van de Ruit, A.M.B.C. de · Voskamp-Visser, I. · Hooft, P.A.V. van · Mars-Groenendijk, R.H. · Visser, P.C. de · Noort, D.
Type:article
Date:2006
Institution: TNO Defensie en Veiligheid
Source:Chemical Biology and Drug Design, 3, 68, 148-153
Identifier: 239455
doi: doi:10.1111/j.1747-0285.2006.00424.x
Keywords: Antimicrobial peptide · Biological agents · DnaK · Drosocin · Lipopolysaccharide · Spectrum · Dosocin · Lipopolysaccharide · Protein DnaK · Amino acid sequence · Antibacterial activity · Antibiotic sensitivity · Article · Bacterial strain · Controlled study · Enterobacteriaceae · Gram negative bacterium · Micrococcus luteus · Minimum inhibitory concentration · Nonhuman · Priority journal · Salmonella · Sequence homology · Xanthomonas campestris · Amino Acid Sequence · Anti-Bacterial Agents · Antimicrobial Cationic Peptides · Drosophila Proteins · Glycopeptides · Gram-Negative Bacteria · Gram-Positive Bacteria · Microbial Sensitivity Tests · Bacteria (microorganisms) · Drosophila melanogaster · Enterobacteriaceae · Micrococcus luteus · Negibacteria · Salmonella · Xanthomonas campestris

Abstract

Drosocin is a 19-mer, cationic antimicrobial peptide from Drosophila melanogaster. The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a panel of 31 bacterial strains, including Salmonella strains with truncated lipopolysaccharide structures, was tested for susceptibility towards three drosocin analogues. Available bacterial DnaK amino acid sequences were retrieved from the ExPASy proteomics server of the Swiss Institute of Bioinformatics studied for sequence homology. Seventeen of the 31 strains tested were susceptible for the drosocin analogues. Minimal inhibitory concentration values against mainly Gram-negative bacteria ranged from 3.1 to 100 μm. With the exception of Micrococcus luteus and Xanthomonas campestris all drosocin analogue-susceptible strains were Enterobacteriaceae showing a high DnaK amino acid sequence homology. © 2006 The Authors. Chemicals / CAS: drosocin, 179048-25-0; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; drosocin, 149924-99-2; Drosophila Proteins; Glycopeptides